imveliso

Iidosi ezinkulu ze-aflatoxins zikhokelela kwityhefu ebukhali (i-aflatoxicosis) enokubeka ubomi esichengeni, ngokwesiqhelo ngomonakalo esibindini.
I-Aflatoxin B1 yi-aflatoxin eveliswa yi-Aspergillus flavus kunye ne-A. parasiticus.Yi-carcinogen enamandla kakhulu.La mandla e-carcinogenic ayohluka kwiintlobo ngeentlobo nezinye, ezinje ngeempuku kunye neenkawu, zibonakala zichaphazeleka ngakumbi kunezinye.I-Aflatoxin B1 yingcoliseko eqhelekileyo kwiindidi zokutya okuquka amandongomane, i-cottonseed meal, umbona, kunye nezinye iinkozo;ngokunjalo nokutya kwezilwanyana.I-Aflatoxin B1 ithathwa njengeyona ityhefu ye-aflatoxin kwaye ibandakanyeka kakhulu kwi-hepatocellular carcinoma (HCC) ebantwini.[cating] Kwizilwanyana, i-aflatoxin B1 ikwabonakaliswe njenge-mutagenic, i-teratogenic, kwaye ibangela ukugonywa komzimba.Iisampulu ezininzi kunye neendlela zohlalutyo ezibandakanya i-chromatography (TLC), i-high-performance liquid chromatography (HPLC), i-mass spectrometry, kunye ne-enzyme-linked immunosorbent assay (ELISA), phakathi kwezinye, zisetyenziselwe ukuvavanya ukungcoliseka kwe-aflatoxin B1 ekutyeni. .Ngokutsho kwe-Food and Agriculture Organisation (FAO), amanqanaba aphezulu anyanyezelwayo e-aflatoxin B1 emhlabeni wonke axelwe ukuba akuluhlu lwe-1-20 μg/kg ekutyeni, kunye ne-5-50 μg/kg kwisondlo senkomo esityayo ngo-2003.

Iinkcukacha

1.Elisa Test Kit ye-Aflatoxin B1

2.Ikati.KA07202H-96wells

3. Amacandelo eKit
● I-Microtiter plateprecoated ne-antigen, amaqula angama-96
● Isisombululo esisemgangathweni × 6 ibhotile (1ml/ibhotile)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● I-Enzyme conjugate 7ml………………………………………………………………………………
● Isicombululo se-antibody7ml............................................... ...................................................……ikepusi eluhlaza
● I-Substrate A 7ml……………………………………………………………………………………ikepusi emhlophe
● I Substrate B 7ml……………….……………………………………………………………………
● Misa isisombululo 7ml……….……………………………………………………………………………………
● 20×isisombululo esigxininisiweyo sokuhlamba 40ml ………………………………………………
● 2×isisombululo esigxininisiweyo sotsalo 50ml………………………………………………………

4.Uvakalelo, ukuchaneka nokuchaneka
Uvakalelo: 0.05ppb

5.Umda wokufunyanwa
Isampulu yeoli etyiwayo.............................................. .................................................. .......................0.1ppb
Peanut................................................. .................................................. .......................0.2ppb
Icereal................................................. .................................................. .....................0.05ppb
Ukuchaneka
Isampulu yeoli etyiwayo.............................................. .................................................. ....................80±15%
Peanut................................................. .................................................. ....................80±15%
Icereal................................................. .................................................. ....................80±15%
Ukuchaneka:Ukwahluka komlinganiso wekhithi ye-ELISA ingaphantsi kwe-10%.

6.Ireyithi enqamlezileyo
IAflatoxin B1 ·················100%
Aflatoxin B2 ······················81 .3%.
I-Aflatoxin G1 ·······················62%
Aflatoxin G2 ·······················22.3%.