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Elisa Test Kit of Ochratoxin A Featured Image
  • U-Elisa woVavanyo lweKit ye-Ochratoxin A
  • U-Elisa woVavanyo lweKit ye-Ochratoxin A

U-Elisa woVavanyo lweKit ye-Ochratoxin A

Inkcazelo emfutshane:

Le khithi ingasetyenziswa kuhlalutyo lobungakanani kunye nomgangatho we-ochratoxin A kwisondlo.Yimveliso entsha yokufunyanwa kwentsalela yechiza esekwe kwitekhnoloji ye-ELISA, exabisa kuphela i-30min kumsebenzi ngamnye kwaye inokunciphisa kakhulu iimpazamo zokusebenza kunye nokuqina komsebenzi.Le khithi isekelwe kubuchwephesha obungathanga ngqo be-ELISA.Imithombo ye-microtiter ifakwe kwi-antigen yokudibanisa.I-Ochratoxin A kwisampulu ikhuphisana ne-antigen egqunywe kwipleyiti ye-microtiter ye-antibody eyongeziweyo.Emva kokongezwa kwe-enzyme conjugate, i-substrate ye-TMB isetyenziselwa ukubonisa umbala.I-Absorbance yesampuli inxulumene kakubi ne-o chratoxin A intsalela kuyo, emva kokuthelekisa ne-Standard Curve, iphindwe yi-dilution factor, i-Ochratoxin A ubuninzi kwisampulu ingabalwa.


Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ii-Ochratoxins liqela lee-mycotoxins eziveliswa zezinye iintlobo ze-Aspergillus (ubukhulu becala A).I-Ochratoxin A yaziwa ngokuba ivela kwiimveliso ezifana neesiriyeli, ikofu, iziqhamo ezomisiweyo kunye newayini ebomvu.Ithathwa njenge-carcinogen yomntu kwaye inomdla okhethekileyo njengoko inokuqokelelwa kwinyama yezilwanyana.Ngaloo ndlela inyama kunye neemveliso zenyama zinokungcoliswa yile tyhefu.Ukuvezwa kwi-ochratoxins ngokutya kunokuba netyhefu ebukhali kwizintso ezincancisayo, kwaye inokuba yi-carcinogenic.

Iinkcukacha

1. IKithi yoVavanyo luka-Elisa we-Ochratoxin A

2. Ikati.KA07301H-96 Wells

3. Amacandelo eKit
● Icwecwe leMicrotiter elinamaqula angama-96 aqatywe ngeantigen
● Izisombululo eziqhelekileyo (iibhotile ezi-6: 1ml/ibhotile)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● I-Enzyme conjugate 7ml…………………………………………………………………..………..…..icap ebomvu
● Isisombululo se-antibody 10ml………………………………………………………………………….…
● Isisombululo sesubstrate A 7ml ……………………………………………………………………………
● ISubstrate Solution B 7ml……………………………………………………………..………………………
● Misa isisombululo 7ml …………………………………………………………………………………………………
● 20×isisombululo esigxininisiweyo seWash 40ml………..…………………………………….…………

4. Uvakalelo, ukuchaneka nokuchaneka
Uvakalelo loVavanyo: 0.4ppb
Umda wokufunyanwa
Ukutya …………………………………………………………………………………….…
Ukuchaneka
Ukutya ……………………………………………………………………….….…90±20%
Ukuchaneka:Ukwahluka komlinganiso wekhithi ye-ELISA ingaphantsi kwe-10%.

5. Inqanaba lokuwela
Ochratoxin A……………………………………………………………………..100%

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